Brian Bosire

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Portfolio project using github pages.

View the Project on GitHub Itsbosire/Port-folio

About me

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Biotechnologitst | Bioinformatician | Molecular Biologist

I am a passionate biotechnologist, molecular biologist, and bioinformatician with strong enthusiasm for bridging lab science and computational analysis. My work involves applying molecular tools, exploring genetic mechanisms, and using data-driven approaches to solve challenges in health, agriculture, and biotechnology.

Research Interests

Education

Cholera Epidemiology Project Overview Designed a cholera epidemiology surveillance dashboard using R to track and visualize cholera outbreaks, enhancing public health response strategies. Skills & Tools: R shiny, Epidemiology, Data wrangling and visualization

Link to dashboard

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Phylogenetic Analysis and Comparative Genomics ClusterVenn Conducted comprehensive phylogenetic analysis and comparative genomics of Puccinia graminis f.s.p isolates, providing insights into their evolutionary relationships and pathogenicity. Tools: Bash, FastQC, MultiQC, Fastp, Busco, Quast, Augustus, EggNog Mapper, Orthofinder, MAFFT, iTol, Fasttree, MuMmer, Snippy

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MRSA Genome Assembly MRSA Stapled a complete genome assembly of Methicillin-resistant Staphylococcus aureus (MRSA) using Illumina sequencing data, enhancing understanding of its genetic makeup and resistance mechanisms. Tools: FastQC, Fastp, Spades, Quast

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MTB Variant Calling Pipeline MTB Developed a robust NGS variant calling pipeline for Mycobacterium tuberculosis (MTB) using bash scripting, automating the analysis of sequencing data to identify genetic variants associated with drug resistance. Tools: FastQC, MultiQC, Fastp, BWA, Samtools, freebayes

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To view more of my projects, please visit my GitHub profile.

Skills

Bioinformatics: Genome Assembly, Variant Calling, Phylogenetics, Comparative Genomics, Molecular Docking, NGS Data Analysis, Sequence Alignment, Data Visualization

Programming Languages: Python, R, Bash

Laboratory Techniques: PCR, Gel Electrophoresis, DNA/RNA Extraction, Spectrophotometry, Microscopy

Tools & Software: Bioconductor, Galaxy, MEGA, AutoDock Vina , PyMOL, FastQC, Trimmomatic, BWA, SAMtools, GATK, IGV, RStudio, Git/GitHub,velvet, SPAdes, Prokka, BLAST, ClustalW, MAFFT,Orthofinder, IQ-TREE, FigTree, VMD, Discovery Studio

Soft Skills: Problem-Solving, Critical Thinking, Communication, Teamwork, Adaptability, Time Management

Contact Me

I am always open to connecting with fellow researchers, professionals, and enthusiasts in the fields of biotechnology, molecular biology, and bioinformatics. Reach out to me via email, phone, or social media for collaborations, discussions, or opportunities.

email

phone

Twitter

GitHub

Location

Eldoret , Kenya.